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R&D Systems mouse anti netrin 1
Mouse Anti Netrin 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+netrin+1/pm41612432-52-8-11?v=R%26D+Systems
Average 94 stars, based on 31 article reviews

mouse anti netrin 1 - by Bioz Stars, 2026-07

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(A) Experimental scheme showing the transduction of Adenovirus-Cre or -empty to pancreatic organoids isolated from LSL-Kras G12D/+ mice. d, day. Adeno, adenovirus. Scale bar, 20 μm. (B) qRT-PCR for axon guidance molecules and neurotrophins using the pancreatic organoids. n = 7 mice. A.U., arbitrary units. Two-tailed unpaired Student’s t-tests. (C) qRT-PCR for Ntn1 using pancreatic tissues from wild-type (WT), Pdx1-Cre; LSL-Kras G12D/+ (KC), and Pdx1-Cre; LSL-Kras G12D/+ ; LSL-Trp53 R172H/+ (KPC) mice. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (D) Co-immunofluorescence (Co-IF) for NTN1 and CK19 (a duct marker) and quantification of the ratio of NTN1 + cells in CK19 + ductal cells. Normal pancreas from WT mice and pancreatic intraepithelial neoplasia (PanIN1, 2, and 3) and pancreatic ductal adenocarcinoma (PDAC) areas from KPC mice were evaluated. The inset shows a magnified view of normal ductal cells lacking NTN1 + signals. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (E) qRT-PCR for NTN1 receptors using pancreatic tissues from WT, KC, and KPC mice. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (F) Co-immunofluorescence for NEO1 and CK19 and quantification of the ratio of NEO1 + cells in CK19 + ductal cells. Normal pancreas from WT mice and PanIN and PDAC areas from KPC mice were evaluated. The inset shows a magnified view of normal ductal cells lacking NEO1 + signals. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (G) Co-immunofluorescence for NTN1 and CK19 using human normal pancreas and PDAC tissues. CK19 + ductal areas are magnified in the insets. n = 12 patients with PDAC. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (H) The expression of NTN1 and NEO1 in bulk RNA-sequencing data using human pancreatic tissues. n = 165 (normal) and 178 patients (PDAC). The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) datasets were analyzed. FPKM, fragments per kilobase of transcript per million mapped reads. Two-tailed unpaired Student’s t-tests. In all figures, bar graphs show mean ± s.e.m (standard error of the mean), and asterisks denote the following P-values. ****, P-value < 0.0001; ***, P-value of 0.0001 to 0.001; **, P-value of 0.001 to 0.01; *, P-value of 0.01 to 0.05; ns, P-value ≥ 0.05. A , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: (A) Experimental scheme showing the transduction of Adenovirus-Cre or -empty to pancreatic organoids isolated from LSL-Kras G12D/+ mice. d, day. Adeno, adenovirus. Scale bar, 20 μm. (B) qRT-PCR for axon guidance molecules and neurotrophins using the pancreatic organoids. n = 7 mice. A.U., arbitrary units. Two-tailed unpaired Student’s t-tests. (C) qRT-PCR for Ntn1 using pancreatic tissues from wild-type (WT), Pdx1-Cre; LSL-Kras G12D/+ (KC), and Pdx1-Cre; LSL-Kras G12D/+ ; LSL-Trp53 R172H/+ (KPC) mice. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (D) Co-immunofluorescence (Co-IF) for NTN1 and CK19 (a duct marker) and quantification of the ratio of NTN1 + cells in CK19 + ductal cells. Normal pancreas from WT mice and pancreatic intraepithelial neoplasia (PanIN1, 2, and 3) and pancreatic ductal adenocarcinoma (PDAC) areas from KPC mice were evaluated. The inset shows a magnified view of normal ductal cells lacking NTN1 + signals. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (E) qRT-PCR for NTN1 receptors using pancreatic tissues from WT, KC, and KPC mice. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (F) Co-immunofluorescence for NEO1 and CK19 and quantification of the ratio of NEO1 + cells in CK19 + ductal cells. Normal pancreas from WT mice and PanIN and PDAC areas from KPC mice were evaluated. The inset shows a magnified view of normal ductal cells lacking NEO1 + signals. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (G) Co-immunofluorescence for NTN1 and CK19 using human normal pancreas and PDAC tissues. CK19 + ductal areas are magnified in the insets. n = 12 patients with PDAC. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (H) The expression of NTN1 and NEO1 in bulk RNA-sequencing data using human pancreatic tissues. n = 165 (normal) and 178 patients (PDAC). The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) datasets were analyzed. FPKM, fragments per kilobase of transcript per million mapped reads. Two-tailed unpaired Student’s t-tests. In all figures, bar graphs show mean ± s.e.m (standard error of the mean), and asterisks denote the following P-values. ****, P-value < 0.0001; ***, P-value of 0.0001 to 0.001; **, P-value of 0.001 to 0.01; *, P-value of 0.01 to 0.05; ns, P-value ≥ 0.05. A , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Article Snippet: Immunohistochemistry for NTN1 and NEO1 were performed using the chicken polyclonal anti-NTN1 antibody (NB100-1605, Novus, 1:200, RRID: AB_2298756) and the goat polyclonal anti-NEO1 antibody (AF1079, R&D, 1:200, RRID: AB_2151002). (A) Experimental scheme showing the transduction of Adenovirus-Cre or -empty to pancreatic organoids isolated from LSL-Kras G12D/+ mice. d, day.

Techniques: Transduction, Isolation, Quantitative RT-PCR, Two Tailed Test, Comparison, Immunofluorescence, Marker, Expressing, RNA Sequencing

(A) qRT-PCR for Ntn1 and Neo1 using murine LSL-Kras G12D/+ pancreatic organoids transduced with Adenovirus-Cre or -Empty. See Fig. 1A for the experimental schematic. 24-hour treatment with the MEK inhibitor trametinib (10 nM) or vehicle was performed 6 days after adenovirus transduction. n = 6 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (B) Co-immunofluorescence for NTN1, phosphorylated ERK (pERK), and CK19. Normal pancreas from WT mice and pancreatic intraepithelial neoplasia (PanIN1, 2, and 3) from KC mice were evaluated. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Statistical analysis was performed using the percentage of NTN1 + pERK + cells in total CK19 + cells (red). n = 4 mice each. Scale bars, 50 μm. (C) Experimental scheme showing the isolation of pancreatic organoids from KC mice and treatment of the KC organoids with vehicle, isoproterenol (ISO; β agonist; 0.1 μM), ICI118,551 (ICI; β2-specific antagonist; 10 μM), and trametinib (10 nM) for 6 days. Scale bar, 20 μm. (D) qRT-PCR for Ntn1 and Neo1 using KC pancreatic organoids treated with the indicated drugs. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (E) Experimental scheme showing in vivo treatment of KC mice (16-20 weeks of age) with vehicle, ISO (10 μg/g/day), and ISO + ICI (0.2 μg/g/day). These drugs were intraperitoneally injected into KC mice daily for 6 days. (F) Co-immunofluorescence for NTN1 (upper panel) and NEO1 (lower panel) with CK19 using pancreatic tissues from KC mice treated with vehicle, ISO, or ISO+ICI. n = 5 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 50 μm. C and E , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: (A) qRT-PCR for Ntn1 and Neo1 using murine LSL-Kras G12D/+ pancreatic organoids transduced with Adenovirus-Cre or -Empty. See Fig. 1A for the experimental schematic. 24-hour treatment with the MEK inhibitor trametinib (10 nM) or vehicle was performed 6 days after adenovirus transduction. n = 6 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (B) Co-immunofluorescence for NTN1, phosphorylated ERK (pERK), and CK19. Normal pancreas from WT mice and pancreatic intraepithelial neoplasia (PanIN1, 2, and 3) from KC mice were evaluated. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Statistical analysis was performed using the percentage of NTN1 + pERK + cells in total CK19 + cells (red). n = 4 mice each. Scale bars, 50 μm. (C) Experimental scheme showing the isolation of pancreatic organoids from KC mice and treatment of the KC organoids with vehicle, isoproterenol (ISO; β agonist; 0.1 μM), ICI118,551 (ICI; β2-specific antagonist; 10 μM), and trametinib (10 nM) for 6 days. Scale bar, 20 μm. (D) qRT-PCR for Ntn1 and Neo1 using KC pancreatic organoids treated with the indicated drugs. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (E) Experimental scheme showing in vivo treatment of KC mice (16-20 weeks of age) with vehicle, ISO (10 μg/g/day), and ISO + ICI (0.2 μg/g/day). These drugs were intraperitoneally injected into KC mice daily for 6 days. (F) Co-immunofluorescence for NTN1 (upper panel) and NEO1 (lower panel) with CK19 using pancreatic tissues from KC mice treated with vehicle, ISO, or ISO+ICI. n = 5 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 50 μm. C and E , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Techniques: Mutagenesis, Quantitative RT-PCR, Transduction, Comparison, Immunofluorescence, Isolation, In Vivo, Injection

(A and B) Celiac Ganglia (CG) were isolated from Th-Cre/Rosa26-tdtomato mice, and the CG tissue explants were treated with recombinant NTN1 (rNTN1; 100 ng/mL) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL) for 7 days. (A) Experimental scheme. (B) Representative images and quantification of tdtomato + neurite outgrowth. White dotted lines indicate the areas of neurite elongation. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (C) Experimental schematic showing epithelial knockout of Ntn1 in the KC mouse model. M, months. (D) Percentage of PanIN lesions in the total pancreatic tissue area was evaluated using hematoxylin and eosin (H&E)-stained sections from the pancreas of Ntn1 -WT KC and Ntn1 -cKO (conditional KO) KC mice. Green areas denote PanIN lesions. n = 5 mice each. KCN, Pdx1-Cre; LSL-Kras G12D ; Ntn1 flox/flox . Two-tailed unpaired Student’s t-tests. Scale bars, 1 mm. (E) Whole-mount staining for tyrosine hydroxylase (TH) was performed using optically cleared pancreatic tissue from WT, KC, and KCN mice at 12 months of age. Autofluorescence was used to visualize the pancreatic structure. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 1 mm. (F ) Experimental scheme showing the injection of the retrograde neuronal tracer Fast Blue into the pancreas of WT, KC, and KCN mice. (G) Fast Blue (FB) fluorescence and TH immunofluorescence signals were evaluated using CGs from WT, KC, and KCN mice 1 week after FB injection. Areas surrounded by yellow dotted lines indicate the CG tissues. Red arrowheads denote neurons double-positive for TH and FB. n = 4 mice each. See Supplementary Fig. S3J for images from the individual channels (TH and FB). One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 50 μm. A, C, and F , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: (A and B) Celiac Ganglia (CG) were isolated from Th-Cre/Rosa26-tdtomato mice, and the CG tissue explants were treated with recombinant NTN1 (rNTN1; 100 ng/mL) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL) for 7 days. (A) Experimental scheme. (B) Representative images and quantification of tdtomato + neurite outgrowth. White dotted lines indicate the areas of neurite elongation. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (C) Experimental schematic showing epithelial knockout of Ntn1 in the KC mouse model. M, months. (D) Percentage of PanIN lesions in the total pancreatic tissue area was evaluated using hematoxylin and eosin (H&E)-stained sections from the pancreas of Ntn1 -WT KC and Ntn1 -cKO (conditional KO) KC mice. Green areas denote PanIN lesions. n = 5 mice each. KCN, Pdx1-Cre; LSL-Kras G12D ; Ntn1 flox/flox . Two-tailed unpaired Student’s t-tests. Scale bars, 1 mm. (E) Whole-mount staining for tyrosine hydroxylase (TH) was performed using optically cleared pancreatic tissue from WT, KC, and KCN mice at 12 months of age. Autofluorescence was used to visualize the pancreatic structure. n = 4 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 1 mm. (F ) Experimental scheme showing the injection of the retrograde neuronal tracer Fast Blue into the pancreas of WT, KC, and KCN mice. (G) Fast Blue (FB) fluorescence and TH immunofluorescence signals were evaluated using CGs from WT, KC, and KCN mice 1 week after FB injection. Areas surrounded by yellow dotted lines indicate the CG tissues. Red arrowheads denote neurons double-positive for TH and FB. n = 4 mice each. See Supplementary Fig. S3J for images from the individual channels (TH and FB). One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 50 μm. A, C, and F , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Techniques: Isolation, Recombinant, Blocking Assay, Comparison, Knock-Out, Staining, Two Tailed Test, Injection, Fluorescence, Immunofluorescence

(A) KC organoids (pancreatic organoids isolated from Pdx1-Cre; LSL-Kras G12D/+ mice) were treated with recombinant NTN1 (rNTN1; 100 ng/mL) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL) for 6 days. The number and area of organoids were evaluated. n = 3 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (B) qRT-PCR for Zeb1 and Vim using sg-control- and sg- Neo1 -expressing mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. n = 3 each. sg, single guide RNA. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. ( C) Western blotting for ZEB1, VIM, and SOX9 using sg-ctrl (control) and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. ( D) Sphere assay using sg-ctrl and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 6 days. Arrowheads denote cancer spheres. n = 5 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (E) qRT-PCR for Sox9 , Sox2, Cd44, and Prom1 using sg-ctrl and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (F) mT4 organoids were treated with rNTN1 (100 ng/mL), NEO1-blocking antibody (NEO1 Ab; 500 ng/mL), and FAK inhibitor (FAKi; defactinib; 1 μM) for 6 days. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (G) qRT-PCR for Zeb1, Vim, and Sox9 using mT4 organoids treated with rNTN1 (100 ng/mL), NEO1 Ab (500 ng/mL), and FAKi (defactinib; 1 μM) for 48 hours. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (H) Kaplan-Meier survival curves. n = 16 (KPC) and 15 mice (KPCN). w, weeks. Log-rank test. (I) Co-immunofluorescence for phosphorylated FAK (pFAK) and CK19 using pancreatic tumors from KPC and KPCN mice. n = 6 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (J-K) Co-immunofluorescence for ZEB1 (J) and SOX9 (K) with CK19 using pancreatic tumors from KPC and KPCN mice. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 4 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 50 μm.

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: (A) KC organoids (pancreatic organoids isolated from Pdx1-Cre; LSL-Kras G12D/+ mice) were treated with recombinant NTN1 (rNTN1; 100 ng/mL) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL) for 6 days. The number and area of organoids were evaluated. n = 3 mice each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (B) qRT-PCR for Zeb1 and Vim using sg-control- and sg- Neo1 -expressing mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. n = 3 each. sg, single guide RNA. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. ( C) Western blotting for ZEB1, VIM, and SOX9 using sg-ctrl (control) and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. ( D) Sphere assay using sg-ctrl and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 6 days. Arrowheads denote cancer spheres. n = 5 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (E) qRT-PCR for Sox9 , Sox2, Cd44, and Prom1 using sg-ctrl and sg- Neo1 mT4 cells treated with rNTN1 (100 ng/mL) for 48 hours. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (F) mT4 organoids were treated with rNTN1 (100 ng/mL), NEO1-blocking antibody (NEO1 Ab; 500 ng/mL), and FAK inhibitor (FAKi; defactinib; 1 μM) for 6 days. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. Scale bars, 100 μm. (G) qRT-PCR for Zeb1, Vim, and Sox9 using mT4 organoids treated with rNTN1 (100 ng/mL), NEO1 Ab (500 ng/mL), and FAKi (defactinib; 1 μM) for 48 hours. n = 4 each. One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests. (H) Kaplan-Meier survival curves. n = 16 (KPC) and 15 mice (KPCN). w, weeks. Log-rank test. (I) Co-immunofluorescence for phosphorylated FAK (pFAK) and CK19 using pancreatic tumors from KPC and KPCN mice. n = 6 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (J-K) Co-immunofluorescence for ZEB1 (J) and SOX9 (K) with CK19 using pancreatic tumors from KPC and KPCN mice. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 4 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 50 μm.

Techniques: Activation Assay, Isolation, Recombinant, Blocking Assay, Comparison, Quantitative RT-PCR, Control, Expressing, Western Blot, Immunofluorescence, Two Tailed Test

(A) Experimental schematic showing a PDAC liver metastasis model generated by splenic injection of NTN1-overexpressing (NTN1-OE) and control mT4 cells. w, weeks. (B) Kaplan-Meier survival curves. n = 8 mice each. Log-rank test. (C) Histological tumor areas were evaluated using livers collected 2 weeks after splenic injection. Green areas denote tumor regions. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (D) Whole-mount staining for PGP9.5 was performed using optically cleared liver tissues. n = 4 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (E) Immunofluorescence for TH using mT4 liver metastasis. Red arrowheads denote TH + adrenergic nerves. n = 4 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (F and G) Co-immunofluorescence for ZEB1 (F) and SOX9 (G) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. A , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: (A) Experimental schematic showing a PDAC liver metastasis model generated by splenic injection of NTN1-overexpressing (NTN1-OE) and control mT4 cells. w, weeks. (B) Kaplan-Meier survival curves. n = 8 mice each. Log-rank test. (C) Histological tumor areas were evaluated using livers collected 2 weeks after splenic injection. Green areas denote tumor regions. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (D) Whole-mount staining for PGP9.5 was performed using optically cleared liver tissues. n = 4 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (E) Immunofluorescence for TH using mT4 liver metastasis. Red arrowheads denote TH + adrenergic nerves. n = 4 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. (F and G) Co-immunofluorescence for ZEB1 (F) and SOX9 (G) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 50 μm. A , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Techniques: Generated, Injection, Control, Two Tailed Test, Staining, Immunofluorescence

Blockade of Ntn1 or Neo1 inhibits the features of EMT and cancer stemness, and restrains the progression of PDAC liver metastasis. (A) Experimental scheme showing a liver metastasis model generated by splenic injection of control (sg-control) and Neo1 -knockout mT4 cells which express sg Neo1 #1. w, weeks. (B) Kaplan-Meier survival curves. n = 10 mice each. Log-rank test. (C) Histological assessment of tumor area was performed 2 weeks after splenic injection. Green areas denote tumor areas. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (D and E) Co-immunofluorescence for ZEB1 (D) and SOX9 (E) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 100 μm. (F) Experimental scheme showing a liver metastasis model generated by splenic injection of mT4 cells and treatment of tumor-bearing mice with a NTN1-neutralizing antibody (NTN1 Ab; NP137) or isotype antibody. Mice were intraperitoneally injected with NTN1 Ab (10 mg/kg) or isotype IgG antibody every other day. (G) Kaplan-Meier survival curves. n = 7 mice each. Log-rank test. (H) Histological assessment of tumor areas was performed 2 weeks after splenic injection. Green areas denote tumor areas. n = 7 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (I and J) Co-immunofluorescence for ZEB1 (I) and SOX9 (J) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 100 μm. A and F , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Journal: Cancer research

Article Title: Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1

doi: 10.1158/0008-5472.CAN-25-2243

Figure Lengend Snippet: Blockade of Ntn1 or Neo1 inhibits the features of EMT and cancer stemness, and restrains the progression of PDAC liver metastasis. (A) Experimental scheme showing a liver metastasis model generated by splenic injection of control (sg-control) and Neo1 -knockout mT4 cells which express sg Neo1 #1. w, weeks. (B) Kaplan-Meier survival curves. n = 10 mice each. Log-rank test. (C) Histological assessment of tumor area was performed 2 weeks after splenic injection. Green areas denote tumor areas. n = 5 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (D and E) Co-immunofluorescence for ZEB1 (D) and SOX9 (E) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 100 μm. (F) Experimental scheme showing a liver metastasis model generated by splenic injection of mT4 cells and treatment of tumor-bearing mice with a NTN1-neutralizing antibody (NTN1 Ab; NP137) or isotype antibody. Mice were intraperitoneally injected with NTN1 Ab (10 mg/kg) or isotype IgG antibody every other day. (G) Kaplan-Meier survival curves. n = 7 mice each. Log-rank test. (H) Histological assessment of tumor areas was performed 2 weeks after splenic injection. Green areas denote tumor areas. n = 7 mice each. Two-tailed unpaired Student’s t-test. Scale bars, 2 mm. (I and J) Co-immunofluorescence for ZEB1 (I) and SOX9 (J) with CK19 using mT4 liver metastasis. The nuclear ZEB1 + or SOX9 + area in CK19 + cells was quantified. n = 5 mice each. Two-tailed unpaired Student’s t-tests. Scale bars, 100 μm. A and F , Created in BioRender. Wu, F. (2025) https://BioRender.com/mlrkrl8

Techniques: Generated, Injection, Control, Knock-Out, Two Tailed Test, Immunofluorescence

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